From biological event to
measurable evidence.

Preserve structures in blood, identify targets by their molecular markers, and measure their abundance and morphology.

How the platform works.

From preserved blood to structure-resolved NETosis data.

  1. Preserve the sample
  2. Label selected markers
  3. Image the population
  4. Measure individual events
Explore the data

One sample.
Evidence at every scale.

Explore a whole-blood sample, then select an event to compare DNA, H3Cit and MPO signals within the same structure.

Inspect the complete gel stripe.

ZOOM

Event Gallery.

Select any H3Cit-associated event to inspect its DAPI, SYTOX, H3Cit and MPO channels.

Event 047

MERGE + ROI
DAPI
SYTOX
H3Cit
MPO
Relative signal within this gallery
DAPIP89
SYTOXP58
H3CitP40
MPOP26

Compare these events with curated cellular phenotypes and larger extracellular structures.

Explore the NETosis Atlas

From events to population profiles.

Generated example data show how event counts, marker signals and structure size contribute to a sample profile.

Sample signature

The higher-burden example relative to the lower-burden example.

A generated example profile ordered from H3Cit-positive target frequency through MPO confirmation, extracellular-DNA expansion, large-event fraction and integrated NETosis burden. The vertical line marks the lower-burden example, with relative values scaled for comparison.LOWER-BURDEN EXAMPLEH3Cit-positive target frequencyH3Cit+ frequencyMPO-confirmed target fractionMPO+ fractionExtracellular-DNA expansionDNA expansionLarge-event fractionLarge-event fractionIntegrated NETosis burdenIntegrated burdenLOWERHIGHERRELATIVE PROFILE

Compare the populations

Both examples use the same assumed sample size and axes.

Lower-burden example

5,000 H3Cit-selected targets.

5,000 H3Cit-selected targets. Every point is one H3Cit-selected target. Horizontal position represents H3Cit intensity, vertical position represents the SYTOX-to-DAPI area ratio, color represents MPO colocalization and point size represents event area.LOWERHIGHERH3Cit SIGNAL INTENSITYSYTOX:DAPI AREA RATIO

Higher-burden example

9,000 H3Cit-selected targets.

9,000 H3Cit-selected targets. Every point is one H3Cit-selected target. Horizontal position represents H3Cit intensity, vertical position represents the SYTOX-to-DAPI area ratio, color represents MPO colocalization and point size represents event area.LOWERHIGHERH3Cit SIGNAL INTENSITYSYTOX:DAPI AREA RATIO
ONE H3Cit-SELECTED TARGETMPO COLOCALIZATION · LOW / MID / HIGHEVENT AREA
ReadoutLower burdenHigher burden
H3Cit+ targets5,0009,000
MPO-confirmed70.7%70.3%
Qualified events3,5366,323
Mean event area1.0×1.9×
DNA expansion1.0×1.9×
Large-event fraction1.3%30.1%
Integrated burden1.0×3.4×

How the readouts are calculated

In these generated examples, qualified events have mid/high MPO colocalization. Integrated burden sums their normalized event areas; large events have normalized area ≥ 0.50.

DNA expansion, mean area and integrated burden are shown relative to the lower-burden example. Both populations assume the same sample size.

Panels built around
the biology.

Extend the marker panel beyond DNA, H3Cit and MPO to investigate cell lineage, pathway state, molecular cargo or cell–NET interactions. Targets and interpretation criteria are defined for each study.

Registered images

Whole-sample views and multichannel images of individual events, with measurements traceable to the source images.

Quality checks and measurements

Processing quality checks and study-defined readouts of event abundance, morphology, marker localization and interactions.

Research report

Methods, results and interpretation limits brought together with the agreed endpoint tables.

Discuss your study